Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF3 / XPLN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124825-100
Catalog Number: LS-G124825
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF3 (NM_001128615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 59.8 kDA protein | DKFZP434F2429 | GEF3 | STA3 | RhoGEF protein | ARHGEF3 | XPLN
- Origin Species
- Human
- NCBI GenBank
- NM_019555
- SwissProt
- Q9NR81
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSSTAMNQC SCRGMEENKE RPKRQRQNNF PMFPSPKAWN FRGRKRKQST QDEDAVSLCS61LDISEPSNKR VKPLSRVTSL ANLIPPVKAT PLKRFSQTLQ RSISFRSESR PDILAPRPWS121RNAAPSSTKR RDSKLWSETF DVCVNQMLTS KEIKRQEAIF ELSQGEEDLI EDLKLAKKAY181HDPMLKLSIM TEQELNQIFG TLDSLIPLHE ELLSQLRDVR KPDGSTEHVG PILVGWLPCL241SSYDSYCSNQ VAAKALLDHK KQDHRVQDFL QRCLESPFSR KLDLWNFLDI PRSRLVKYPL301LLREILRHTP NDNPDQQHLE EAINIIQGIV AEINTKTGES ECRYYKERLL YLEEGQKDSL361IDSSRVLCCH GELKNNRGVK LHVFLFQEVL VITRAVTHNE QLCYQLYRQP IPVKDLLLED421LQDGEVRLGG SLRGAFSNNE RIKNFFRVSF KNGSQSQTHS LQANDTFNKQ QWLNCIRQAK481ETVLCAAGQA GVLDSEGSFL NPTTGSRELQ GETKLEQMDQ SDSESDCSMD TSEVSLDCER541MEQTDSSCGN SRHGESNVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
558 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.