Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF19 / WGEF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105874-100
Catalog Number: LS-G105874
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF19 (NM_153213), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF19 | Ephexin-2 | Weakly similar to Rho GEF | WGEF | RP4-733M16.1
- Origin Species
- Human
- SwissProt
- Q8IW93
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDCGPPATLQ PHLTGPPGTA HHPVAVCQQE SLSFAELPAL KPPSPVCLDL FPVAPEELRA61PGSRWSLGTP APLQGLLWPL SPGGSDTEIT SGGMRPSRAG SWPHCPGAQP PALEGPWSPR121HTQPQRRASH GSEKKSAWRK MRVYQREEVP GCPEAHAVFL EPGQVVQEQA LSTEEPRVEL181SGSTRVSLEG PERRRFSASE LMTRLHSSLR LGRNSAARAL ISGSGTGAAR EGKASGMEAR241SVEMSGDRVS RPAPGDSREG DWSEPRLDTQ EEPPLGSRST NERRQSRFLL NSVLYQEYSD301VASARELRRQ QREEEGPGDE AEGAEEGPGP PRANLSPSSS FRAQRSARGS TFSLWQDIPD361VRGSGVLATL SLRDCKLQEA KFELITSEAS YIHSLSVAVG HFLGSAELSE CLGAQDKQWL421FSKLPEVKST SERFLQDLEQ RLEADVLRFS VCDVVLDHCP AFRRVYLPYV TNQAYQERTY481QRLLLENPRF PGILARLEES PVCQRLPLTS FLILPFQRIT RLKMLVENIL KRTAQGSEDE541DMATKAFNAL KELVQECNAS VQSMKRTEEL IHLSKKIHFE GKIFPLISQA RWLVRHGELV601ELAPLPAAPP AKLKLSSKAV YLHLFNDCLL LSRRKELGKF AVFVHAKMAE LQVRDLSLKL661QGIPGHVFLL QLLHGQHMKH QFLLRARTES EKQRWISALC PSSPQEDKEV ISEGEDCPQV721QCVRTYKALH PDELTLEKTD ILSVRTWTSD GWLEGVRLAD GEKGWVPQAY VEEISSLSAR781LRNLRENKRV TSATSKLGEA PVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
802 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.