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ARHGEF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF15 antibodies →
$504.00each
Catalog Number: LS-G105301-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rho guanine exchange factor 15(ARHGEF15) (NM_173728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARGEF15 | ARHGEF15 | E5 | Ephexin-5 | Ephexin5 | Rho GEF 15 | Vsm-RhoGEF | KIAA0915
Origin Species
Human
NCBI GenBank
AB020722
SwissProt
O94989

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAQSLPAAT PPTQKPPRII RPRPPSRSRA AQSPGPPHNG SSPQELPRNS NDAPTPMCTP
61IFWEPPAASL KPPALLPPSA SRASLDSQTS PDSPSSTPTP SPVSRRSASP EPAPRSPVPP
121PKPSGSPCTP LLPMAGVLAQ NGSASAPGTV RRLAGRFEGG AEGRAQDADA PEPGLQARAD
181VNGEREAPLT GSGSQENGAP DAGLACPPCC PCVCHTTRPG LELRWVPVGG YEEVPRVPRR
241ASPLRTSRSR PHPPSIGHPA VVLTSYRSTA ERKLLPLLKP PKPTRVRQDA TIFGDPPQPD
301LDLLSEDGIQ TGDSPDEAPQ NTPPATVEGR EEEGLEVLKE QNWELPLQDE PLYQTYRAAV
361LSEELWGVGE DGSPSPANAG DAPTFPRPPG PRNTLWQELP AVQASGLLDT LSPQERRMQE
421SLFEVVTSEA SYLRSLRLLT DTFVLSQALR DTLTPRDHHT LFSNVQRVQG VSERFLATLL
481SRVRSSPHIS DLCDVVHAHA VGPFSVYVDY VRNQQYQEET YSRLMDTNVR FSAELRRLQS
541LPKCERLPLP SFLLLPFQRI TRLRMLLQNI LRQTEEGSSR QENAQKALGA VSKIIERCSA
601EVGRMKQTEE LIRLTQRLRF HKVKALPLVS WSRRLEFQGE LTELGCRRGG VLFASRPRFT
661PLCLLLFSDL LLITQPKSGQ RLQVLDYAHR SLVQAQQVPD PSGPPTFRLS LLSNHQGRPT
721HRLLQASSLS DMQRWLGAFP TPGPLPCSPD TIYEDCDCSQ ELCSESSAPA KTEGRSLESR
781AAPKHLHKTP EGWLKGLPGA FPAQLVCEVT GEHERRRHLR QNQRLLEAVG SSSGTPNAPP
841P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

841 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.