LSBio
ARHGEF11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF11 antibodies →
$504.00each
Catalog Number: LS-G103726-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF11 (NM_198236), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTRAP48 | PDZ-RHOGEF | ARHGEF11 | KIAA0380
Origin Species
Human
NCBI GenBank
NM_014784
SwissProt
O15085

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
172.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVRLPQSID RLSSLSSLGD SAPERKSPSH HRQPSDASET TGLVQRCVII QKDQHGFGFT
61VSGDRIVLVQ SVRPGGAAMK AGVKEGDRII KVNGTMVTNS SHLEVVKLIK SGAYVALTLL
121GSSPSSMGIS GLQQDPSPAG APRITSVIPS PPPPPPLPPP QRITGPKPLQ DPEVQKHATQ
181ILRNMLRQEE KELQRICEVY SRNPASLLEE QIEGARRRVT QLQLKIQQET GGSVDILPLY
241GDTSQRPSEG RLSLDSQEGD SGLDSGTERF PSLSESLMNR NSVLSDPGLD SPRTSPVIMA
301RVAQHHRRQG SDAAVPSTGD QGVDQSPKPL IIGPEEDYDP GYFNNESDII FQDLEKLKSR
361PAHLGVFLRY IFSQADPSPL LFYLCAEVYQ QASPKDSRSL GKDIWNIFLE KNAPLRVKIP
421EMLQAEIDSR LRNSEDARGV LCEAQEAAMP EIQEQIHDYR TKRTLGLGSL YGENDLLDLD
481GDPLRERQVA EKQLAALGDI LSKYEEDRSA PMDFALNTYM SHAGIRLREA RPSNTAEKAQ
541SAPDKDKWLP FFPKTKKSSN SKKEKDALED KKRNPILKYI GKPKSSSQST FHIPLSPVEV
601KPGNVRNIIQ HFENNQQYDA PEPGTQRLST GSFPEDLLES DSSRSEIRLG RSESLKGREE
661MKRSRKAENV PRSRSDVDMD AAAEATRLHQ SASSSTSSLS TRSLENPTPP FTPKMGRRSI
721ESPSLGFCTD TLLPHLLEDD LGQLSDLEPE PDAQNWQHTV GKDVVAGLTQ REIDRQEVIN
781ELFVTEASHL RTLRVLDLIF YQRMKKENLM PREELARLFP NLPELIEIHN SWCEAMKKLR
841EEGPIIKEIS DLMLARFDGP AREELQQVAA QFCSYQSIAL ELIKTKQRKE SRFQLFMQEA
901ESHPQCRRLQ LRDLIISEMQ RLTKYPLLLE SIIKHTEGGT SEHEKLCRAR DQCREILKYV
961NEAVKQTENR HRLEGYQKRL DATALERASN PLAAEFKSLD LTTRKMIHEG PLTWRISKDK
1021TLDLHVLLLE DLLVLLQKQD EKLLLKCHSK TAVGSSDSKQ TFSPVLKLNA VLIRSVATDK
1081RAFFIICTSK LGPPQIYELV ALTSSDKNTW MELLEEAVRN ATRHPGAAPM PVHPPPPGPR
1141EPAQQGPTPS RVELDDSDVF HGEPEPEELP GGTGSQQRVQ GKHQVLLEDP EQEGSAEEEE
1201LGVLPCPSTS LDGENRGIRT RNPIHLAFPG PLFMEGLADS ALEDVENLRH LILWSLLPGH
1261TMETQAAQEP EDDLTPTPSV ISVTSHPWDP GSPGQAPPGG EGDNTQLAGL EGERPEQEDM
1321GLCSLEHLPP RTRNSGIWES PELDRNLAED ASSTEAAGGY KVVRKAEVAG SKVVPALPES
1381GQSEPGPPEV EGGTKATGNC FYVSMPSGPP DSSTDHSEAP MSPPQPDSLP AGQTEPQPQL
1441QGGNDDPRRP SRSPPSLALR DVGMIFHTIE QLTLKLNRLK DMELAHRELL KSLGGESSGG
1501TTPVGSFHTE AARWTDGSLS PPAKEPLASD SRNSHELGPC PEDGSDAPLE DSTADAAASP
1561GP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.