LSBio
ARHGEF10L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF10L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF10L antibodies →
$725.00each
Catalog Number: LS-G111985-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF10L (NM_018125), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF10L | GrinchGEF | RP11-473A10.1 | KIAA1626
Origin Species
Human
NCBI GenBank
NM_018125
SwissProt
Q9HCE6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
140.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASSNPPPQP AIGDQLVPGV PGPSSEAEDD PGEAFEFDDS DDEEDTSAAL GVPSLAPERD
61TDPPLIHLDS IPVTDPDPAA APPGTGVPAW VSNGDAADAA FSGARHSSWK RKSSRRIDRF
121TFPALEEDVI YDDVPCESPD AHQPGAERNL LYEDAHRAGA PRQAEDLGWS SSEFESYSED
181SGEEAKPEVE VEPAKHRVSF QPKLSPDLTR LKERYARTKR DILALRVGGR DMQELKHKYD
241CKMTQLMKAA KSGTKDGLEK TRMAVMRKVS FLHRKDVLGD SEEEDMGLLE VSVSDIKPPA
301PELGPMPEGL SPQQVVRRHI LGSIVQSEGS YVESLKRILQ DYRNPLMEME PKALSARKCQ
361VVFFRVKEIL HCHSMFQIAL SSRVAEWDST EKIGDLFVAS FSKSMVLDVY SDYVNNFTSA
421MSIIKKACLT KPAFLEFLKR RQVCSPDRVT LYGLMVKPIQ RFPQFILLLQ DMLKNTPRGH
481PDRLSLQLAL TELETLAEKL NEQKRLADQV AEIQQLTKSV SDRSSLNKLL TSGQRQLLLC
541ETLTETVYGD RGQLIKSKER RVFLLNDMLV CANINFKPAN HRGQLEISSL VPLGPKYVVK
601WNTALPQVQV VEVGQDGGTY DKDNVLIQHS GAKKASASGQ AQNKVYLGPP RLFQELQDLQ
661KDLAVVEQIT LLISTLHGTY QNLNMTVAQD WCLALQRLMR VKEEEIHSAN KCRLRLLLPG
721KPDKSGRPIS FMVVFITPNP LSKISWVNRL HLAKIGLREE NQPGWLCPDE DKKSKAPFWC
781PILACCIPAF SSRALSLQLG ALVHSPVNCP LLGFSAVSTS LPQGYLWVGG GQEGAGGQVE
841IFSLNRPSPR TVKSFPLAAP VLCMEYIPEL EEEAESRDES PTVADPSATV HPTICLGLQD
901GSILLYSSVD TGTQCLVSCR SPGLQPVLCL RHSPFHLLAG LQDGTLAAYP RTSGGVLWDL
961ESPPVCLTVG PGPVRTLLSL EDAVWASCGP RVTVLEATTL QPQQSFEAHQ DEAVSVTHMV
1021KAGSGVWMAF SSGTSIRLFH TETLEHLQEI NIATRTTFLL PGQKHLCVTS LLICQGLLWV
1081GTDQGVIVLL PVPRLEGIPK ITGKGMVSLN GHCGPVAFLA VATSILAPDI LRSDQEEAEG
1141PRAEEDKPDG QAHEPMPDSH VGRELTRKKG ILLQYRLRST AHLPGPLLSM REPAPADGAA
1201LEHSEEDGSI YEMADDPDIW VRSRPCARDA HRKEICSVAI ISGGQGYRNF GSALGSSGRQ
1261APCGETDSTL LIWQVPLML
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1279 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.