Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF10 / GEF10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113814-100
Catalog Number: LS-G113814
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF10 (NM_014629), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF10 | GEF10 | KIAA0294
- Origin Species
- Human
- NCBI GenBank
- NM_014629
- SwissProt
- O15013
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 148.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQREPLPPA PAENEMKYDT NNNEEEEGEQ FDFDSGDEIP EADRQAPSAP ETGGAGASEA61PAPTGGEDGA GAETTPVAEP TKLVLPMKVN PYSVIDITPF QEDQPPTPVP SAEEENVGLH121VPCGYLVPVP CGYAVPSNLP LLLPAYSSPV IICATSLDEE ETPEVTEDRQ PNSLSSEEPP181TSEDQVGRED SALARWAADP ANTAWMENPE EAIYDDVPRE NSDSEPDEMI YDDVENGDEG241GNSSLEYGWS SSEFESYEEQ SDSECKNGIP RSFLRSNHKK QLSHDLTRLK EHYEKKMRDL301MASTVGVVEI QQLRQKHELK MQKLVKAAKD GTKDGLERTR AAVKRGRSFI RTKSLIAQDH361RSSLEEEQNL FIDVDCKHPE AILTPMPEGL SQQQVVRRYI LGSVVDSEKN YVDALKRILE421QYEKPLSEME PKVLSERKLK TVFYRVKEIL QCHSLFQIAL ASRVSEWDSV EMIGDVFVAS481FSKSMVLDAY SEYVNNFSTA VAVLKKTCAT KPAFLEFLKQ EQEASPDRTT LYSLMMKPIQ541RFPQFILLLQ DMLKNTSKGH PDRLPLQMAL TELETLAEKL NERKRDADQR CEVKQIAKAI601NERYLNKLLS SGSRYLIRSD DMIETVYNDR GEIVKTKERR VFMLNDVLMC ATVSSRPSHD661SRVMSSQRYL LKWSVPLGHV DAIEYGSSAG TGEHSRHLAV HPPESLAVVA NAKPNKVYMG721PGQLYQDLQN LLHDLNVIGQ ITQLIGNLKG NYQNLNQSVA HDWTSGLQRL ILKKEDEIRA781ADCCRIQLQL PGKQDKSGRP TFFTAVFNTF TPAIKESWVN SLQMAKLALE EENHMGWFCV841EDDGNHIKKE KHPLLVGHMP VMVAKQQEFK IECAAYNPEP YLNNESQPDS FSTAHGFLWI901GSCTHQMGQI AIVSFQNSTP KVIECFNVES RILCMLYVPV EEKRREPGAP PDPETPAVRA961SDVPTICVGT EEGSISIYKS SQGSKKVRLQ HFFTPEKSTV MSLACTSQSL YAGLVNGAVA1021SYARAPDGSW DSEPQKVIKL GVLPVRSLLM MEDTLWAASG GQVFIISVET HAVEGQLEAH1081QEEGMVISHM AVSGVGIWIA FTSGSTLRLF HTETLKHLQD INIATPVHNM LPGHQRLSVT1141SLLVCHGLLM VGTSLGVLVA LPVPRLQGIP KVTGRGMVSY HAHNSPVKFI VLATALHEKD1201KDKSRDSLAP GPEPQDEDQK DALPSGGAGS SLSQGDPDAA IWLGDSLGSM TQKSDLSSSS1261GSLSLSHGSS SLEHRSEDST IYDLLKDPVS LRSKARRAKK AKASSALVVC GGQGHRRVHR1321KARQPHQEEL APTVMVWQIP LLNITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1344 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.