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ARHGAP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP9 antibodies →
$725.00each
Catalog Number: LS-G120173-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP9 (NM_001080156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
10C | ARHGAP9
Origin Species
Human
NCBI GenBank
NM_032496
SwissProt
Q9BRR9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEPPVYCNL VDLRRCPRSP PPGPACPLLQ RLDAWEQHLD PNSGRCFYIN SLTGCKSWKP
61PRRSRSETNP GSMEGTQTLK RNNDVLQPQA KGFRSDTGTP EPLDPQGSLS LSQRTSQLDP
121PALQAPRPLP QLLDDPHEVE KSGLLNMTKI AQGGRKLRKN WGPSWVVLTG NSLVFYREPP
181PTAPSSGWGP AGSRPESSVD LRGAALAHGR HLSSRRNVLH IRTIPGHEFL LQSDHETELR
241AWHRALRTVI ERLDRENPLE LRLSGSGPAE LSAGEDEEEE SELVSKPLLR LSSRRSSIRG
301PEGTEQNRVR NKLKRLIAKR PPLQSLQERG LLRDQVFGCQ LESLCQREGD TVPSFLRLCI
361AAVDKRGLDV DGIYRVSGNL AVVQKLRFLV DRERAVTSDG RYVFPEQPGQ EGRLDLDSTE
421WDDIHVVTGA LKLFLRELPQ PLVPPLLLPH FRAALALSES EQCLSQIQEL IGSMPKPNHD
481TLRYLLEHLC RVIAHSDKNR MTPHNLGIVF GPTLFRPEQE TSDPAAHALY PGQLVQLMLT
541NFTSLFP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

547 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.