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ARHGAP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP6 antibodies →
$725.00each
Catalog Number: LS-G114262-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP6 (NM_013423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP6 | RHOGAPX-1 | RHOGAP6
Origin Species
Human
NCBI GenBank
NM_001174

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGRSVRLRS VPIQSLSELE RARLQEVAFY QLQQDCDLSC QITIPKDGQK RKKSLRKKLD
61SLGKEKNKDK EFIPQAFGMP LSQVIANDRA YKLKQDLQRD EQKDASDFVA SLLPFGNKRQ
121NKELSSSNSS LSSTSETPNE STSPNTPEPA PRARRRGAMS VDSITDLDDN QSRLLEALQL
181SLPAEAQSKK EKARDKKLSL NPIYRQVPRL VDSCCQHLEK HGLQTVGIFR VGSSKKRVRQ
241LREEFDRGID VSLEEEHSVH DVAALLKEFL RDMPDPLLTR ELYTAFINTL LLEPEEQLGT
301LQLLIYLLPP CNCDTLHRLL QFLSIVARHA DDNISKDGQE VTGNKMTSLN LATIFGPNLL
361HKQKSSDKEF SVQSSARAEE STAIIAVVQK MIENYEALFM VPPDLQNEVL ISLLETDPDV
421VDYLLRRKAS QSSSPDMLQS EVSFSVGGRH SSTDSNKASS GDISPYDNNS PVLSERSLLA
481MQEDAAPGGS EKLYRVPGQF MLVGHLSSSK SRESSPGPRL GKDLSEEPFD IWGTWHSTLK
541SGSKDPGMTG SSGDIFESSS LRAGPCSLSQ GNLSPNWPRW QGSPAELDSD TQGARRTQAA
601APATEGRAHP AVSRACSTPH VQVAGKAERP TARSEQYLTL SGAHDLSESE LDVAGLQSRA
661TPQCQRPHGS GRDDKRPPPP YPGPGKPAAA AAWIQGPPEG VETPTDQGGQ AAEREQQVTQ
721KKLSSANSLP AGEQDSPRLG DAGWLDWQRE RWQIWELLST DNPDALPETL V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

771 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.