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ARHGAP40 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP40 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP40 antibodies →
$725.00each
Catalog Number: LS-G128307-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP40 (NM_001164431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ1100H13.4 | ARHGAP40 | C20orf95
Origin Species
Human
NCBI GenBank
NM_001164431
SwissProt
Q5TG30

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEPALLPAA QMERLAPGPL ASPCPRIPRA RIARRCAQRW ADLGCSSGPS SGRMDQLPQK
61NLLRLHPAGS AGCSTGVESS SMDGFWMEVE QIQQRDELRE EDSGGNEGQL PEGEAESQWL
121QDTGLSGLLG GLGLDGDHQE LLSTLTQTQV AAVCRRLDIY ARSVRRQHKT PVRDVRDVFG
181VFNSGKMSSE NGDSGMKGAQ LSSGASKFPP AAEPGGLQEQ AGREEAFNMD SAYSEQAAVL
241LQRSRPSRGG TSAWGKCSLP KFTVPKGRLG VTRIGDLSLQ DMRKVPSLAL IELTALCDIL
301GLDLKRSKAG KWKAAETRLF GVPLDSLLEA DHKVLPSTQV PLVLQALLSC LEKRGLDMEG
361ILRVPGSQAR VKGLEQKLER DFYAGLFSWD EVHHNDASDL LKRFIRKLPT PLLTAEYLPA
421FAVVPNIPNL KQRLQVLHLL ILILPEPNRN ALKALLEFLR KVVAREQHNK MTLRNVSTVM
481APNLFLHQGR PPKLPKGKEK QLAEGAAEVV QIMVHYQDLL WTVASFLVAQ VRKLNDSSSR
541RPQLCDAGLK TWLRRMHADR DKAGDGLEAT PKVAKIQVVW PIKDPLKVPL TPSTKVAHVL
601RQFTEHLSPG SKGQEDSEDM DSLLLHHRSM ESANILLYEV GGNINEHRLD PDAYLLDLYR
661ANPHGEWVLK QNPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.