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ARHGAP39 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP39 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP39 antibodies →
$725.00each
Catalog Number: LS-G109504-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1688(ARHGAP39) (NM_025251), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CrGAP | CrossGAP homolog | KIAA1688 | Vilse | ARHGAP39 | RhoGAP93B homolog
Origin Species
Human
SwissProt
Q9C0H5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQTQDYECR SHNVDLPESR IPGSNTRLEW VEIIEPRTRE RMYANLVTGE CVWDPPAGVR
61IKRTSENQWW ELFDPNTSRF YYYNASTQRT VWHRPQGCDI IPLAKLQTLK QNTESPRASA
121ESSPGRGSSV SREGSTSSSL EPEPDTEKAQ ELPARAGRPA AFGTVKEDSG SSSPPGVFLE
181KDYEIYRDYS ADGQLLHYRT SSLRWNSGAK ERMLIKVADR EPSFLAAQGN GYAPDGPPGV
241RSRRPSGSQH SPSLQTFAPE ADGTIFFPER RPSPFLKRAE LPGSSSPLLA QPRKPSGDSQ
301PSSPRYGYEP PLYEEPPVEY QAPIYDEPPM DVQFEAGGGY QAGSPQRSPG RKPRPFLQPN
361KQGPPSPCQQ LVLTKQKCPE RFLSLEYSPA GKEYVRQLVY VEQAGSSPKL RAGPRHKYAP
421NPGGGSYSLQ PSPCLLRDQR LGVKSGDYST MEGPELRHSQ PPTPLPQAQE DAMSWSSQQD
481TLSSTGYSPG TRKRKSRKPS LCQATSATPT EGPGDLLVEQ PLAEEQPPCG TSLAPVKRAE
541GEAEGARGAA EPFLAQARLA WEAQQAHFHM KQRSSWDSQQ DGSGYESDGA LPLPMPGPVV
601RAFSEDEALA QQENRHWRRG TFEKLGFPQI LLEKSVSVQT NLASPEPYLH PSQSEDLAAC
661AQFESSRQSR SGVPSSSCVF PTFTLRKPSS ETDIENWASK HFNKHTQGLF RRKVSIANML
721AWSSESIKKP MIVTSDRHVK KEACELFKLI QMYMGDRRAK ADPLHVALEV ATKGWSVQGL
781RDELYIQLCR QTTENFRLES LARGWELMAI CLAFFPPTPK FHSYLEGYIY RHMDPVNDTK
841VTQHIKELLE RNTKKKSKLR KKPKPYVEEP DGVAISTYAK YCYHKLQKAA LTGAKKGLKK
901PNVEEIRHAK NAVFSPSMFG SALQEVMGMQ RERYPERQLP WVQTRLSEEV LALNGDQTEG
961IFRVPGDIDE VNALKLQVDQ WKVPTGLEDP HVPASLLKLW YRELEEPLIP HEFYEQCIAH
1021YDSPEAAVAV VHALPRINRM VLCYLIRFLQ VFVQPANVAV TKMDVSNLAM VMAPNCLRCQ
1081SDDPRVIFEN TRKEMSFLRV LIQHLDTSFM EGVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1114 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.