Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGAP36 / FLJ30058 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106790-100
Catalog Number: LS-G106790
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP36 (NM_144967), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGAP36 | FLJ30058 | RP13-102H20.1
- Origin Species
- Human
- SwissProt
- Q6ZRI8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGCIPFLKA ARALCPRIMP PLLLLSAFIF LVSVLGGAPG HNPDRRTKMV SIHSLSELER61LKLQETAYHE LVARHFLSEF KPDRALPIDR PNTLDKWFLI LRGQQRAVSH KTFGISLEEV121LVNEFTRRKH LELTATMQVE EATGQAAGRR RGNVVRRVFG RIRRFFSRRR NEPTLPREFT181RRGRRGAVSV DSLAELEDGA LLLQTLQLSK ISFPIGQRLL GSKRKMSLNP IAKQIPQVVE241ACCQFIEKHG LSAVGIFTLE YSVQRVRQLR EEFDQGLDVV LDDNQNVHDV AALLKEFFRD301MKDSLLPDDL YMSFLLTATL KPQDQLSALQ LLVYLMPPCH SDTLERLLKA LHKITENCED361SIGIDGQLVP GNRMTSTNLA LVFGSALLKK GKFGKRESRK TKLGIDHYVA SVNVVRAMID421NWDVLFQVPP HIQRQVAKRV WKSSPEALDF IRRRNLRKIQ SARIKMEEDA LLSDPVETSA481EARAAVLAQS KPSDEGSSEE PAVPSGTARS HDDEEGAGNP PIPEQDRPLL RVPREKEAKT541GVSYFFPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
547 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.