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ARHGAP30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP30 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP30 antibodies →
$504.00each
Catalog Number: LS-G104394-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP30 (NM_181720), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP11-544M22.6 | ARHGAP30
Origin Species
Human
NCBI GenBank
H59365
SwissProt
Q7Z6I6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKSRQKGKKK GSAKERVFGC DLQEHLQHSG QEVPQVLKSC AEFVEEYGVV DGIYRLSGVS
61SNIQKLRQEL ESERKPDLRR DVYLQDIHCV SSLCKAYFRE LPDPLLTYRL YDKFAEAVGV
121QLEPERLVKI LEVLRELPVP NYRTLEFLMR HLVHMASFSA QTNMHARNLA IVWAPNLLRS
181KDIEASGFNG TAAFMEVRVQ SIVVEFILTH VDQLFGGAAL SGGEVESGWR SLPGTRASGS
241PEDLMPRPLP YHLPSILQAG DGPPQMRPYH TIIEIAEHKR KGSLKVRKWR SIFNLGRSGH
301ETKRKLPRGA EDREDKSNKG TLRPAKSMDS LSAAAGASDE PEGLVGPSSP RPSPLLPESL
361ENDSIEAAEG EQEPEAEALG GTNSEPGTPR AGRSAIRAGG SSRAERCAGV HISDPYNVNL
421PLHITSILSV PPNIISNVSL ARLTRGLECP ALQHRPSPAS GPGPGPGLGP GPPDEKLEAS
481PASSPLADSG PDDLAPALED SLSQEVQDSF SFLEDSSSSE PEWVGAEDGE VAQAEAAGAA
541FSPGEDDPGM GYLEELLGVG PQVEEFSVEP PLDDLSLDEA QFVLAPSCCS VDSAGPRPEV
601EEENGEEVFL SAYDDLSPLL GPKPPIWKGS GSLEGEAAGC GRQALGQGGE EQACWEVGED
661KQAEPGGRLD IREEAEGSPQ PPQPEEMEPE GQPSPDGCLC PCSLGLGGVG MRLASTLVQV
721QQVRSVPVVP PKPQFAKMPS AMCSKIHVAP ANPCPRPGRL DGTPGERAWG SRASRSSWRN
781GGSLSFDAAV ALARDRQRTE AQGVRRTQTC TEGGDYCLIP RTSPCSMISA HSPRPLSCLE
841LPSEGAEGSG SRSRLSLPPR EPQVPDPLLS SQRRSYAFET QANPGKGEGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

890 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.