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ARHGAP28 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP28 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP28 antibodies →
$725.00each
Catalog Number: LS-G121686-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP28 (NM_001010000), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP28 | KIAA1314
Origin Species
Human
SwissProt
Q9P2N2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRKKDKQSIR DVRDIFGVSE SPPRDTCGNH TNQLDGTKEE RELPRVIKTS GSMPDDASLN
61STTLSDASQD KEGSFAVPRS DSVAILETIP VLPVHSNGSP EPGQPVQNAI SDDDFLEKNI
121PPEAEELSFE VSYSEMVTEA LKRNKLKKSE IKKEDYVLTK FNVQKTRFGL TEAGDLSAED
181MKKIRHLSLI ELTAFFDAFG IQLKRNKTEK VKGRDNGIFG VPLTVLLDGD RKKDPGVKVP
241LVLQKFFEKV EESGLESEGI FRLSGCTAKV KQYREELDAK FNADKFKWDK MCHREAAVML
301KAFFRELPTS LFPVEYIPAF ISLMERGPHV KVQFQALHLM VMALPDANRD AAQALMTFFN
361KVIANESKNR MSLWNISTVM APNLFFSRSK HSDYEELLLA NTAAHIIRLM LKYQKILWKV
421PSFLITQVRR MNEATMLLKK QLPSVRKLLR RKTLERETAS PKTSKVLQKS PSARRMSDVP
481EGVIRVHAPL LSKVSMAIQL NNQTKAKDIL AKFQYENSHG SSECIKIQNQ RLYEIGGNIG
541EHCLDPDAYI LDVYRINPQA EWVIKPQQSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.