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ARHGAP24 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP24 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP24 antibodies →
$725.00each
Catalog Number: LS-G109248-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rho GTPase activating protein 24(ARHGAP24) (NM_031305), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP564B1162 | Filamin-A-associated RhoGAP | p73 | p73RhoGAP | RCGAP72 | RhoGAP of 73 kDa | Sarcoma antigen NY-SAR-88 | ARHGAP24 | FILGAP | RC-GAP72
Origin Species
Human
NCBI GenBank
NM_031305
SwissProt
Q8N264

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEDRNSGGC PAGALASTPF IPKTTYRRIK RCFSFRKGIF GQKLEDTVRY EKRYGNRLAP
61MLVEQCVDFI RQRGLKEEGL FRLPGQANLV KELQDAFDCG EKPSFDSNTD VHTVASLLKL
121YLRELPEPVI PYAKYEDFLS CAKLLSKEEE AGVKELAKQV KSLPVVNYNL LKYICRFLDE
181VQSYSGVNKM SVQNLATVFG PNILRPKVED PLTIMEGTVV VQQLMSVMIS KHDCLFPKDA
241ELQSKPQDGV SNNNEIQKKA TMGQLQNKEN NNTKDSPSRQ CSWDKSESPQ RSSMNNGSPT
301ALSGSKTNSP KNSVHKLDVS RSPPLMVKKN PAFNKGSGIV TNGSFSSSNA EGLEKTQTTP
361NGSLQARRSS SLKVSGTKMG THSVQNGTVR MGILNSDTLG NPTNVRNMSW LPNGYVTLRD
421NKQKEQAGEL GQHNRLSTYD NVHQQFSMMN LDDKQSIDSA TWSTSSCEIS LPENSNSCRS
481STTTCPEQDF FGGNFEDPVL DGPPQDDLSH PRDYESKSDH RSVGGRSSRA TSSSDNSETF
541VGNSSSNHSA LHSLVSSLKQ EMTKQKIEYE SRIKSLEQRN LTLETEMMSL HDELDQERKK
601FTMIEIKMRN AERAKEDAEK RNDMLQKEME QFFSTFGELT VEPRRTERGN TIWIQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

655 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.