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ARHGAP20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP20 antibodies →
$725.00each
Catalog Number: LS-G110965-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP20 (NM_020809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP20 | KIAA1391 | RARHOGAP
Origin Species
Human
NCBI GenBank
AA884003
SwissProt
Q9P2F6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAMSPQQET LGGQPGRSSS LTGVSRLAGG SCTKKKMKTL AERRRSAPSL ILDKALQKRP
61TTRDSPSASV DTCTFLSSLV CSNRTLLIDG RAELKRGLQR QERHLFLFND LFVVAKIKYN
121NNFKIKNKIK LTDMWTASCV DEVGEGNTNA MKSFVLGWPT VNFVATFSSP EQKDKWLSLL
181QRYINLEKEK DYPKSIPLKI FAKDIGNCAY SKTITVMNSD TANEVINMSL PMLGITGSER
241DYQLWVNSGK EEAPYPLIGH EYPYGIKMSH LRDSALLTPG SKDSTTPFNL QEPFLMEQLP
301REMQCQFILK PSRLAAAQQL SDSGHKTFKR RRSIINWAFW RGSSTHLDNL PSSPTSPMPG
361QLFGISLPNI CENDNLPKPV LDMLFFLNQK GPLTKGIFRQ SANVKSCREL KEKLNSGVEV
421HLDCESIFVI ASVLKDFLRN IPGSIFSSDL YDHWVSVMDQ GNDEEKINTV QRLLDQLPRA
481NVVLLRYLFG VLHNIEQHSS SNQMTAFNLA VCVAPSILWP PASSSPELEN EFTKKVSLLI
541QFLIENCLRI FGEEITSLFR EVSVRCDTRE NASDISCFQL NDSSYDSLEN ELNEDVDAPC
601SDLVKKLGQG SRSMDSVLTL SDYDLDQPEV EGLLTLSDFD LAHSKDEDVQ MKRPLESKPV
661NILVYTKIPL RDHARAPSAM CTPSYLSTAA ANAAKSLRRH RRCSEPSIDY LDSKLSYLRE
721FYQKKLRKSS CDAILSQKDE DYLKQNQPLQ EEGKTCFKQS LVTGTDVSKK NATTQNTKKK
781SLSGSEGNHV KLFPKSKPVA ISVASYSPMS SQDHSKNQPF DVNTSGYSPP HTADALKGPR
841THRRCSEPNI EDQNRKLTYL RGIYSKKQHK TSCEAGLLHG EEDYLKRHKS LQMEGQKLIN
901QSLVMGIEVG KSSATNQNTE KVLPPRLNLC PRTSYSSLSS PGTSPSGSSV SSQDSAFSQI
961SEHSVFTPTE TSSPIDCTFQ AQRKREDLSP DFSNASHVSG MPGPSSGQAC SRPAYTKKDT
1021MEWHSQMHSV TLHPSTWLRN GVASLKNWSL KKKAKAARPE EEKIASPKGP LEPPPHASGV
1081PEANSLQEEQ KDLPLRAAEG LSPVQSAQRC SSSPFQDSER HCSSPFSLVE SRLKLCMKSH
1141EEIEPGSQSS SGSLPWERAS ASSWTLEDAT SPDSGPTVVC DIEDRYLTKD I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1191 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.