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ARHGAP19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP19 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP19 antibodies →
$725.00each
Catalog Number: LS-G108557-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGH19(ARHGAP19) (NM_032900), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP19 | Putative RhoGAP protein
Origin Species
Human
NCBI GenBank
NM_032900
SwissProt
Q14CB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MATEAQSEGE VPARESGRSD AICSFVICND SSLRGQPIIF NPDFFVEKLR HEKPEIFTEL
61VVSNITRLID LPGTELAQLM GEVDLKLPGG AGPASGFFRS LMSLKRKEKG VIFGSPLTEE
121GIAQIYQLIE YLHKNLRVEG LFRVPGNSVR QQILRDALNN GTDIDLESGE FHSNDVATLL
181KMFLGELPEP LLTHKHFNAH LKIADLMQFD DKGNKTNIPD KDRQIEALQL LFLILPPPNR
241NLLKLLLDLL YQTAKKQDKN KMSAYNLALM FAPHVLWPKN VTANDLQENI TKLNSGMAFM
301IKHSQKLFKA PAYIRECARL HYLGSRTQAS KDDLDLIASC HTKSFQLAKS QKRNRVDSCP
361HQEETQHHTE EALRELFQHV HDMPESAKKK QLIRQFNKQS LTQTPGREPS TSQVQKRARS
421RSFSGLIKRK VLGNQMMSEK KKKNPTPESV AIGELKGTSK ENRNLLFSGS PAVTMTPTRL
481KWSEGKKEGK KGFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

494 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.