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ARHGAP18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP18 antibodies →
$504.00each
Catalog Number: LS-G108204-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP18 (NM_033515), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP18 | BA307O14.2 | MacGAP | SENEX
Origin Species
Human
NCBI GenBank
AA152337

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSWLSSSQGV VLTAYHPSGK DQTVGNSHAK AGEEATSSRR YGQYTMNQES TTIKVMEKPP
61FDRSISQDSL DELSMEDYWI ELENIKKSSE NSQEDQEVVV VKEPDEGELE EEWLKEAGLS
121NLFGESAGDP QESIVFLSTL TRTQAAAVQK RVETVSQTLR KKNKQYQIPD VRDIFAQQRE
181SKETAPGGTE SQSLRTNENK YQGRDDEASN LVGEEKLIPP EETPAPETDI NLEVSFAEQA
241LNQKESSKEK IQKSKGDDAT LPSFRLPKDK TGTTRIGDLA PQDMKKVCHL ALIELTALYD
301VLGIELKQQK AVKIKTKDSG LFCVPLTALL EQDQRKVPGM RIPLIFQKLI SRIEERGLET
361EGLLRIPGAA IRIKNLCQEL EAKFYEGTFN WESVKQHDAA SLLKLFIREL PQPLLSVEYL
421KAFQAVQNLP TKKQQLQALN LLVILLPDAN RDTLKALLEF LQRVIDNKEK NKMTVMNVAM
481VMAPNLFMCH ALGLKSSEQR EFVMAAGTAN TMHLLIKYQK LLWTIPKFIV NQVRKQNTEN
541HKKDKRAMKK LLKKMAYDRE KYEKQDKSTN DADVPQGVIR VQAPHLSKVS MAIQLTEELK
601ASDVLARFLS QESGVAQTLK KGEVFLYEIG GNIGERCLDD DTYMKDLYQL NPNAEWVIKS
661KPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

663 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.