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ARHGAP15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111716-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP15 (NM_018460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP15 | BM046
Origin Species
Human
NCBI GenBank
NM_018460
SwissProt
Q53QZ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQKSTNSDTS VETLNSTRQG TGAVQMRIKN ANSHHDRLSQ SKSMILTDVG KVTEPISRHR
61RNHSQHILKD VIPPLEQLMV EKEGYLQKAK IADGGKKLRK NWSTSWIVLS SRRIEFYKES
121KQQALSNMKT GHKPESVDLC GAHIEWAKEK SSRKNVFQIT TVSGNEFLLQ SDIDFIILDW
181FHAIKNAIDR LPKDSSCPSR NLELFKIQRS SSTELLSHYD SDIKEQKPEH RKSLMFRLHH
241SASDTSDKNR VKSRLKKFIT RRPSLKTLQE KGLIKDQIFG SHLHKVCERE NSTVPWFVKQ
301CIEAVEKRGL DVDGIYRVSG NLATIQKLRF IVNQEEKLNL DDSQWEDIHV VTGALKMFFR
361ELPEPLFPYS FFEQFVEAIK KQDNNTRIEA VKSLVQKLPP PNRDTMKVPF GHLTKIVAKA
421SKNLMSTQSL GIVFGPTLLR AENETGNMAI HMVYQNQIAE LMLSEYSKIF GSEED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

475 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.