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ARHGAP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111812-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP12 (NM_018287), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP12
Origin Species
Human
NCBI GenBank
NM_018287
SwissProt
Q8IWW6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKMADRSGKI IPGQVYIEVE YDYEYEAKDR KIVIKQGERY ILVKKTNDDW WQVKPDENSK
61AFYVPAQYVK EVTRKALMPP VKQVAGLPNN STKIMQSLHL QRSTENVNKL PELSSFGKPS
121SSVQGTGLIR DANQNFGPSY NQGQTVNLSL DLTHNNGKFN NDSHSPKVSS QNRTRSFGHF
181PGPEFLDVEK TSFSQEQSCD SAGEGSERIH QDSESGDELS SSSTEQIRAT TPPNQGRPDS
241PVYANLQELK ISQSALPPLP GSPAIQINGE WETHKDSSGR CYYYNRGTQE RTWKPPRWTR
301DASISKGDFQ NPGDQELLSS EENYYSTSYS QSDSQCGSPP RGWSEELDER GHTLYTSDYT
361NEKWLKHVDD QGRQYYYSAD GSRSEWELPK YNASSQQQRE IIKSRSLDRR LQEPIVLTKW
421RHSTIVLDTN DKESPTASKP CFPENESSPS SPKHQDTASS PKDQEKYGLL NVTKIAENGK
481KVRKNWLSSW AVLQGSSLLF TKTQGSSTSW FGSNQSKPEF TVDLKGATIE MASKDKSSKK
541NVFELKTRQG TELLIQSDND TVINDWFKVL SSTINNQAVE TDEGIEEEIP DSPGIEKHDK
601EKEQKDPKKL RSFKVSSIDS SEQKKTKKNL KKFLTRRPTL QAVREKGYIK DQVFGSNLAN
661LCQRENGTVP KFVKLCIEHV EEHGLDIDGI YRVSGNLAVI QKLRFAVNHD EKLDLNDSKW
721EDIHVITGAL KMFFRELPEP LFTFNHFNDF VNAIKQEPRQ RVAAVKDLIR QLPKPNQDTM
781QILFRHLTRV IENGEKNRMT YQSIAIVFGP TLLKPEKETG NIAVHTVYQN QIVELILLEL
841SSIFGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

846 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.