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ARHGAP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGAP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGAP10 antibodies →
$504.00each
Catalog Number: LS-G109888-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP10 (NM_024605), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP10 | Graf-related protein 2 | GRAF2 | PSGAP | PS-GAP
Origin Species
Human
NCBI GenBank
NM_024605
SwissProt
A1A4S6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT KSLSVAQRKF
61AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ REIMALSVTE TLIKPLEKFR
121KEQLGAVKEE KKKFDKETEK NYSLIDKHLN LSAKKKDSHL QEADIQVEQN RQHFYELSLE
181YVCKLQEIQE RKKFEFVEPM LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE
241GTRSEVEELM NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF
301NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL TMQAFSEEER
361KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR KCISAVETRG INDQGLYRVV
421GVSSKVQRLL SMLMDVKTCN EVDLENSADW EVKTITSALK QYLRSLPEPL MTYELHGDFI
481VPAKSGSPES RVNAIHFLVH KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP
541TLMRPQEETV AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS
601KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA VPGPPGPDKN
661HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS AVTPLSPGSS PFPFSPPATV
721ADKPPESIRS RKARAVYPCE AEHSSELSFE IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ
781NYVKLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.