Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARG99 / OLF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102466-100
Catalog Number: LS-G102466
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMTC1 (NM_175861), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARG99 | OLF | TMTC1 | TMTC1A
- Origin Species
- Human
- NCBI GenBank
- AL832328
- SwissProt
- Q8IUR5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPFYFHAVN IILHCLVTLV LMYTCDKTVF KNRGLAFVTA LLFAVHPIHT EAVAGIVGRA61DVLACLLFLL AFLSYNRSLD QGCVGGSFPS TVSPFFLLLS LFLGTCAMLV KETGITVFGV121CLVYDLFSLS NKQDKSSNGA LCPRSPQQPG SPQPSSLPGH PHRENGKQQR FPHKGAWGGC181HSPLPPEPKS SGFPVSPRAV WSMMRFLTYS YLLAFNVWLL LAPVTLCYDW QVGSIPLVET241IWDMRNLATI FLAVVMALLS LHCLAAFKRL EHKEVLVGLL FLVFPFIPAS NLFFRVGFVV301AERVLYMPSM GYCILFVHGL SKLCTWLNRC GATTLIVSTV LLLLLFSWKT VKQNEIWLSR361ESLFRSGVQT LPHNAKVHYN YANFLKDQGR NKEAIYHYRT ALKLYPRHAS ALNNLGTLTR421DTAEAKMYYQ RALQLHPQHN RALFNLGNLL KSQEKKEEAI TLLKDSIKYG PEFADAYSSL481ASLLAEQERF KEAEEIYQTG IKNCPDSSDL HNNYGVFLVD TGLPEKAVAH YQQAIKLSPS541HHVAMVNLGR LYRSLGENSM AEEWYKRALQ VAHKAEILSP LGALYYNTGR YEEALQIYQE601AAALQPSQRE LRLALAQVLA VMGQTKEAEK MTNHIVSEET GCLECYRLLS AIYSKQENHD661KALDAIDKAL QLKPKDPKVI SELFFTKGNQ LREQNLLDKA FESYRLAVQL NPDQAQAWMN721MGGIQHIKGK YVSARAYYER ALQLVPDSKL LKENLAKLDR LEKRLQEVRE KDQTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
774 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.