Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARA55 / HIC-5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128668-100
Catalog Number: LS-G128668
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIC5(TGFB1I1) (NM_001164719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARA55 | HIC5 | HIC-5 | TSC-5
- Origin Species
- Human
- NCBI GenBank
- NM_015927
- SwissProt
- O43294
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRSGAPKER PAEPLTPPPS YGHQPQTGSG ESSGASGDKD HLYSTVCKPR SPKPAAPAAP61PFSSSSGVLG TGLCELDRLL QELNATQFNI TDEIMSQFPS SKVASGEQKE DQSEDKKRPS121LPSSPSPGLP KASATSATLE LDRLMASLSD FRVQNHLPAS GPTQPPVVSS TNEGSPSPPE181PTGKGSLDTM LGLLQSDLSR RGVPTQAKGL CGSCNKPIAG QVVTALGRAW HPEHFVCGGC241STALGGSSFF EKDGAPFCPE CYFERFSPRC GFCNQPIRHK MVTALGTHWH PEHFCCVSCG301EPFGDEGFHE REGRPYCRRD FLQLFAPRCQ GCQGPILDNY ISALSALWHP DCFVCRECFA361PFSGGSFFEH EGRPLCENHF HARRGSLCAT CGLPVTGRCV SALGRRFHPD HFTCTFCLRP421LTKGSFQERA GKPYCQPCFL KLFGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
444 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.