Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AR / Androgen Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121786-100
Catalog Number: LS-G121786
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Androgen Receptor(AR) (NM_001011645), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AR | Androgen receptor | Dihydrotestosterone receptor | DHTR | AIS | KD | NR3C4 | HUMARA | SBMA | TFM | SMAX1 | HYSP1
- Origin Species
- Human
- NCBI GenBank
- NM_000044
- SwissProt
- P10275
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MILWLHSLET ARDHVLPIDY YFPPQKTCLI CGDEASGCHY GALTCGSCKV FFKRAAEGKQ61KYLCASRNDC TIDKFRRKNC PSCRLRKCYE AGMTLGARKL KKLGNLKLQE EGEASSTTSP121TEETTQKLTV SHIEGYECQP IFLNVLEAIE PGVVCAGHDN NQPDSFAALL SSLNELGERQ181LVHVVKWAKA LPGFRNLHVD DQMAVIQYSW MGLMVFAMGW RSFTNVNSRM LYFAPDLVFN241EYRMHKSRMY SQCVRMRHLS QEFGWLQITP QEFLCMKALL LFSIIPVDGL KNQKFFDELR301MNYIKELDRI IACKRKNPTS CSRRFYQLTK LLDSVQPIAR ELHQFTFDLL IKSHMVSVDF361PEMMAEIISV QVPKILSGKV KPIYFHTQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
388 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.