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AQR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AQR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AQR antibodies →
$725.00each
Catalog Number: LS-G113773-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IBP160(AQR) (NM_014691), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AQR | Aquarius homolog (mouse) | IBP160 | FSAP164 | KIAA0560
Origin Species
Human
NCBI GenBank
AB011132
SwissProt
O60306

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
171.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA
61IRKIMLLEFS QYLENYLWMN YSPEVSSKAY LMSICCMVNE KFRENVPAWE IFKKKPDHFP
121FFFKHILKAA LAETDGEFSL HEQTVLLLFL DHCFNSLEVD LIRSQVQQLI SLPMWMGLQL
181ARLELELKKT PKLRKFWNLI KKNDEKMDPE AREQAYQERR FLSQLIQKFI SVLKSVPLSE
241PVTMDKVHYC ERFIELMIDL EALLPTRRWF NTILDDSHLL VHCYLSNLVR REEDGHLFSQ
301LLDMLKFYTG FEINDQTGNA LTENEMTTIH YDRITSLQRA AFAHFPELYD FALSNVAEVD
361TRESLVKFFG PLSSNTLHQV ASYLCLLPTL PKNEDTTFDK EFLLELLVSR HERRISQIQQ
421LNQMPLYPTE KIIWDENIVP TEYYSGEGCL ALPKLNLQFL TLHDYLLRNF NLFRLESTYE
481IRQDIEDSVS RMKPWQSEYG GVVFGGWARM AQPIVAFTVV EVAKPNIGEN WPTRVRADVT
541INLNVRDHIK DEWEGLRKHD VCFLITVRPT KPYGTKFDRR RPFIEQVGLV YVRGCEIQGM
601LDDKGRVIED GPEPRPNLRG ESRTFRVFLD PNQYQQDMTN TIQNGAEDVY ETFNIIMRRK
661PKENNFKAVL ETIRNLMNTD CVVPDWLHDI ILGYGDPSSA HYSKMPNQIA TLDFNDTFLS
721IEHLKASFPG HNVKVTVEDP ALQIPPFRIT FPVRSGKGKK RKDADVEDED TEEAKTLIVE
781PHVIPNRGPY PYNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI
841YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETEK DFSRYGRVNY
901VLARRIELLE EVKRLQKSLG VPGDASYTCE TAGYFFLYQV MSRWEEYISK VKNKGSTLPD
961VTEVSTFFPF HEYFANAPQP IFKGRSYEED MEIAEGCFRH IKKIFTQLEE FRASELLRSG
1021LDRSKYLLVK EAKIIAMTCT HAALKRHDLV KLGFKYDNIL MEEAAQILEI ETFIPLLLQN
1081PQDGFSRLKR WIMIGDHHQL PPVIKNMAFQ KYSNMEQSLF TRFVRVGVPT VDLDAQGRAR
1141ASLCNLYNWR YKNLGNLPHV QLLPEFSTAN AGLLYDFQLI NVEDFQGVGE SEPNPYFYQN
1201LGEAEYVVAL FMYMCLLGYP ADKISILTTY NGQKHLIRDI INRRCGNNPL IGRPNKVTTV
1261DRFQGQQNDY ILLSLVRTRA VGHLRDVRRL VVAMSRARLG LYIFARVSLF QNCFELTPAF
1321SQLTARPLHL HIIPTEPFPT TRKNGERPSH EVQIIKNMPQ MANFVYNMYM HLIQTTHHYH
1381QTLLQLPPAM VEEGEEVQNQ ETELETEEEA MTVQADIIPS PTDTSCRQET PAFQTDTTPS
1441ETGATSTPEA IPALSETTPT VVGAVSAPAE ANTPQDATSA PEETK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.