Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
APOL1 / Apolipoprotein L (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125756-100
Catalog Number: LS-G125756
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Apolipoprotein L 1(APOL1) (NM_001136540), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Apolipoprotein L-I | APOL-I | Apolipoprotein L | 1 | APOL | APOL1 | FSGS4 | APO-L | Apolipoprotein L1
- Origin Species
- Human
- NCBI GenBank
- NM_003661
- SwissProt
- O14791
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.93 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGAALLRVS VLCIWMSALF LGVGVRAEEA GARVQQNVPS GTDTGDPQSK PLGDWAAGTM61DPESSIFIED AIKYFKEKVS TQNLLLLLTD NEAWNGFVAA AELPRNEADE LRKALDNLAR121QMIMKDKNWH DKGQQYRNWF LKEFPRLKSK LEDNIRRLRA LADGVQKVHK GTTIANVVSG181SLSISSGILT LVGMGLAPFT EGGSLVLLEP GMELGITAAL TGITSSTIDY GKKWWTQAQA241HDLVIKSLDK LKEVKEFLGE NISNFLSLAG NTYQLTRGIG KDIRALRRAR ANLQSVPHAS301ASRPRVTEPI SAESGEQVER VNEPSILEMS RGVKLTDVAP VSFFLVLDVV YLVYESKHLH361EGAKSETAEE LKKVAQELEE KLNILNNNYK ILQADQELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
398 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.