Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
APOER2 / LRP8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112408-100
Catalog Number: LS-G112408
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ApoER2(LRP8) (NM_017522), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Apolipoprotein E receptor 2 | APOER2 | MCI1 | HSZ75190 | ApoE receptor 2 | LRP-8 | LRP8
- Origin Species
- Human
- NCBI GenBank
- NM_004631
- SwissProt
- Q14114
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLPEPGPLR LLALLLLLLL LLLLQLQHLA AAAADPLLGG QGPAKDCEKD QFQCRNERCI61PSVWRCDEDD DCLDHSDEDD CPKKTCADSD FTCDNGHCIH ERWKCDGEEE CPDGSDESEA121TCTKQVCPAE KLSCGPTSHK CVPASWRCDG EKDCEGGADE AGCATSLGTC RGDEFQCGDG181TCVLAIKHCN QEQDCPDGSD EAGCLQGLNE CLHNNGGCSH ICTDLKIGFE CTCPAGFQLL241DQKTCGDIDE CKDPDACSQI CVNYKGYFKC ECYPGYEMDL LTKNCKAAAG KSPSLIFTNR301HEVRRIDLVK RNYSRLIPML KNVVALDVEV ATNRIYWCDL SYRKIYSAYM DKASDPKEQE361VLIDEQLHSP EGLAVDWVHK HIYWTDSGNK TISVATVDGG RRRTLFSRNL SEPRAIAVDP421LRGFMYWSDW GDQAKIEKSG LNGVDRQTLV SDNIEWPNGI TLDLLSQRLY WVDSKLHQLS481SIDFSGGNRK TLISSTDFLS HPFGIAVFED KVFWTDLENE AIFSANRLNG LEISILAENL541NNPHDIVIFH ELKQPRAPDA CELSVQPNGG CEYLCLPAPQ ISSHSPKYTC ACPDTMWLGP601DMKRCYRDAN EDSKMGSTVT AAVIGIIVPI VVIALLCMSG YLIWRNWKRK NTKSMNFDNP661VYRKTTEEED EDELHIGRTA QIGHVYPARV ALSLEDDGLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
700 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.