Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
APOBR / APOB48R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111631-100
Catalog Number: LS-G111631
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human APOB48R(APOBR) (NM_018690), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Apolipoprotein B-100 receptor | Apolipoprotein B48 receptor | APOB48R | ApoB-48R | Apolipoprotein B receptor | Apolipoprotein B-48 receptor | APOB100R | APOBR | Apolipoprotein B100 receptor
- Origin Species
- Human
- NCBI GenBank
- NM_018690
- SwissProt
- Q0VD83
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDFLRLYLPG LHQALRGALD SLGTFVSYLL GDAVPTVERE AQAAEELGVV AVGKTGKIVE61EEAQEDLEGL RGSQNEGAGR LRGPGDDRRH EVGSSAVEQT WGWGDGSSHG SQAERQDSGA121GETAKAARCQ EPSAHLEARK KSKAGSGACQ DRSGQAQERQ ESHEQEVNRE ERLRSWEQEE181EEEEVRAREP GMARGAESEW TWHGETEGKA GAVGPKAAGD NREMEQGVRE ADAGETEEPG241AEGAGKGEEV VVVEKACEST RAWGTWGPGA EPEDWGILGR EEARTTPGRE EARAILDGEE301ARTISGGEEA ETASGGEEAE TASGGEEAGT ASGGEEAGIA SGGEAGTASG GEEAGTASGG361DEAWTTSGKE EADLLGVRQT EYGAVPGERL LEATGKVWVL EEEGDEEREA EVSPFPKQAQ421VLGTERTEEA AESQTAGREA VGGQEAGESF EGQVDLRGKE AEMRQDLGIR ADRARMEELV481QAEEAQEERG SSRDPVAELP SDGEAEGTAD LEATPEARPE EELTGEESEA AQTSCGLLGV541EWGGLTHSVT KGQGPELMGG AQTPTKQPEE REAGEVELMG VLALSKEEQE RSLEAGPRHA601GSVKPEASEA FPGAWENRTR KDMERGNTQE DAADGEQREE EETAGGQTLA AEAEGDRESE661LSEVPEAGGE GLTTQDAGCG TEEGEASVSE NQELDGSTGA DAGPCPSLGE AYARETEDEE721AEADRTSRRG WRLQAVAVGL PDREDAQTGS VAAGIMGGDV VPHISAAGAG EALEGALGQG781WDSKEKEEAA AGEHAGGQEF GLEGSAEEEV TGRGSQVEAF ESREGGPWGG RVEAEESAGA841EDSCGLDPAG SQTARAEGMG AMVEAGGLLE KWTLLEEEAV GWQEREQRED SEGRCGDYHP901EGEAPRLLDA EGLMVTGGRR AEAKETEPES LEHVRGQEEQ PTHQAPAEAA PESVGEAETA961EAMGSARGGA ANSWSEAPLP GSLLDVSVPR SRVHLSRSSS QRRSRPSFRR TPAWEQQEEP1021PAPNPPEEEL SAPEQRPLQL EEPLEPSPLR HDGTPVPARR RPLGHGFGLA HPGMMQELQA1081RLGRPKPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1088 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.