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APLP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

APLP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G125840-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human APLP2 (NM_001142278), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Amyloid protein homolog | Amyloid-like protein 2 | APLP-2 | APLP2 | APPH | CDEI box-binding protein | CDEBP
Origin Species
Human
NCBI GenBank
NM_001642
SwissProt
Q06481

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAATGTAAAA ATGRLLLLLL VGLTAPALAL AGYIEALAAN AGTGFAVAEP QIAMFCGKLN
61MHVNIQTGKW EPDPTGTKSC FETKEEVLQY CQEMYPELQI TNVMEANQRV SIDNWCRRDK
121KQCKSRFVTP FKCLVPPTPL PTNDVDVYFE TSADDNEHAR FQKAKEQLEI RHRNRMDRVK
181KEWEEAELQA KNLPKAERQT LIQHFQAMVK ALEKEAASEK QQLVETHLAR VEAMLNDRRR
241MALENYLAAL QSDPPRPHRI LQALRRYVRA ENKDRLHTIR HYQHVLAVDP EKAAQMKSQV
301MTHLHVIEER RNQSLSLLYK VPYVAQEIQE EIDELLQEQR ADMDQFTASI SETPVDVRVS
361SEESEEIPPF HPFHPFPALP ENEGSGVGEQ DGGLIGAEEK VINSKNKVDE NMVIDETLDV
421KEMIFNAERV GGLEEERESV GPLREDFSLS SSALIGLLVI AVAIATVIVI SLVMLRKRQY
481GTISHGIVEV DPMLTPEERH LNKMQNHGYE NPTYKYLEQM QI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.