Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
APBA3 / MINT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116349-100
Catalog Number: LS-G116349
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Mint3(APBA3) (NM_004886), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Adapter protein X11gamma | APBA3 | Mint3 | MGC:15815 | X11-like 2 protein | X11L2 | Neuron-specific X11L2 protein | Mint-3
- Origin Species
- Human
- NCBI GenBank
- NM_004886
- SwissProt
- O96018
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDFPTISRSP SGPPAMDLEG PRDILVPSED LTPDSQWDPM PGGPGSLSRM ELDESSLQEL61VQQFEALPGD LVGPSPGGAP CPLHIATGHG LASQEIADAH GLLSAEAGRD DLLGLLHCEE121CPPSQTGPEE PLEPAPRLLQ PPEDPDEDSD SPEWVEGASA EQEGSRSSSS SPEPWLETVP181LVTPEEPPAG AQSPETLASY PAPQEVPGPC DHEDLLDGVI FGARYLGSTQ LVSERNPPTS241TRMAQAREAM DRVKAPDGET QPMTEVDLFV STKRIKVLTA DSQEAMMDHA LHTISYTADI301GCVLVLMARR RLARRPAPQD HGRRLYKMLC HVFYAEDAQL IAQAIGQAFA AAYSQFLRES361GIDPSQVGVH PSPGACHLHN GDLDHFSNSD NCREVHLEKR RGEGLGVALV ESGWGSLLPT421AVIANLLHGG PAERSGALSI GDRLTAINGT SLVGLPLAAC QAAVRETKSQ TSVTLSIVHC481PPVTTAIIHR PHAREQLGFC VEDGIICSLL RGGIAERGGI RVGHRIIEIN GQSVVATPHA541RIIELLTEAY GEVHIKTMPA ATYRLLTGQE QPVYLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
575 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.