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APBA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

APBA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G125049-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human APBA2 (NM_001130414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
APBA2 | D15S1518E | Adapter protein X11beta | LIN-10 | MGC:14091 | Mint-2 | MINT2 | X11-BETA | X11-like | HsT16821 | Neuron-specific X11L protein | X11-like protein | X11L
Origin Species
Human
NCBI GenBank
NM_005503
SwissProt
Q99767

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAHRKLESVG SGMLDHRVRP GPVPHSQEPE SEDMELPLEG YVPEGLELAA LRPESPAPEE
61QECHNHSPDG DSSSDYVNNT SEEEDYDEGL PEEEEGITYY IRYCPEDDSY LEGMDCNGEE
121YLAHSAHPVD TDECQEAVEE WTDSAGPHPH GHEAEGSQDY PDGQLPIPED EPSVLEAHDQ
181EEDGHYCASK EGYQDYYPEE ANGNTGASPY RLRRGDGDLE DQEEDIDQIV AEIKMSLSMT
241SITSASEASP EHGPEPGPED SVEACPPIKA SCSPSRHEAR PKSLNLLPEA KHPGDPQRGF
301KPKTRTPEER LKWPHEQVCN GLEQPRKQQR SDLNGPVDNN NIPETKKVAS FPSFVAVPGP
361CEPEDLIDGI IFAANYLGST QLLSERNPSK NIRMMQAQEA VSRVKNSEGD AQTLTEVDLF
421ISTQRIKVLN ADTQETMMDH ALRTISYIAD IGNIVVLMAR RRMPRSASQD CIETTPGAQE
481GKKQYKMICH VFESEDAQLI AQSIGQAFSV AYQEFLRANG INPEDLSQKE YSDIINTQEM
541YNDDLIHFSN SENCKELQLE KHKGEILGVV VVESGWGSIL PTVILANMMN GGPAARSGKL
601SIGDQIMSIN GTSLVGLPLA TCQGIIKGLK NQTQVKLNIV SCPPVTTVLI KRPDLKYQLG
661FSVQNGIICS LMRGGIAERG GVRVGHRIIE INGQSVVATA HEKIVQALSN SVGEIHMKTM
721PAAMFRLLTG QETPLYI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

737 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.