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AP5Z1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AP5Z1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113649-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPG48(AP5Z1) (NM_014855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AP5Z1 | AP-5 complex subunit zeta-1 | SPG48 | Zeta | Zeta5 | KIAA0415
Origin Species
Human
NCBI GenBank
NM_014855
SwissProt
O43299

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFSAGAESLL HQAREIQDEE LKKFCSRICK LLQAEDLGPD TLDSLQRLFL IISATKYSRR
61LEKTCVDLLQ ATLGLPACPE QLQVLCAAIL REMSPSDSLS LAWDHTQNSR QLSLVASVLL
121AQGDRNEEVR AVGQGVLRAL ESRQPEGPSL RHLLPVMAKV VVLSPGTLQE DQATLLSKRL
181VDWLRYASLQ QGLPHSGGFF STPRARQPGP VTEVDGAVAT DFFTVLSSGH RFTDDQWLNV
241QAFSMLRAWL LHSGPEGPGT LDTDDRSEQE GSTLSVISAT SSAGRLLPPR ERLREVAFEY
301CQRLIEQSNR RALRKGDSDL QKACLVEAVL VLDVLCRQDP SFLYRSLSCL KALHGRVRGD
361PASVRVLLPL AHFFLSHGEA AAVDSEAVYQ HLFTRIPVEQ FHSPMLAFEF IQFCRDNLHL
421FSGHLSTLRL SFPNLFKFLA WNSPPLTSEF VALLPALVDA GTALEMLHAL LDLPCLTAVL
481DLQLRSAPAA SERPLWDTSL RAPSCLEAFR DPQFQGLFQY LLRPKASGAT ERLAPLHQLL
541QPMAGCARVA QCAQAVPTLL QAFFSAVTQV ADGSLINQLA LLLLGRSDSL YPAPGYAAGV
601HSVLSSQFLA LCTLKPSLVV ELARDLLEFL GSVNGLCSRA SLVTSVVWAI GEYLSVTYDR
661RCTVEQINKF FEALEALLFE VTQCRPSAAL PRCPPQVVTV LMTTLTKLAS RSQDLIPRAS
721LLLSKMRTLA HSPATSSTHS EEGAEAIRTR ATELLTLLKM PSVAQFVLTP STEVCSPRYH
781RDANTALPLA LRTVSRLVER EAGLMPG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

807 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.