Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AP5M1 / C14orf108 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111885-100
Catalog Number: LS-G111885
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AP5M1 (NM_018229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AP-5 complex subunit mu | AP-5 complex subunit mu-1 | C14orf108 | MuD | Mu-2 related death-inducing | AP5M1 | Mu5 | MUDENG
- Origin Species
- Human
- NCBI GenBank
- NM_018229
- SwissProt
- Q9H0R1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQRAVWLIS HEPGTPLCGT VRFSRRYPTV EKRARVFNGA SYVPVPEDGP FLKALLFELR61LLDDDKDFVE SRDSCSRINK TSIYGLLIGG EELWPVVAFL KNDMIYACVP LVEQTLSPRP121PLISVSGVSQ GFEFLFGIQD FLYSGQKNDS ELNTKLSQLP DLLLQACPFG TLLDANLQNS181LDNTNFASVT QPQKQPAWKT GTYKGKPQVS ISITEKVKSM QYDKQGIADT WQVVGTVTCK241CDLEGIMPNV TISLSLPTNG SPLQDILVHP CVTSLDSAIL TSSSIDAMDD SAFSGPYKFP301FTPPLESFNL CFYTSQVPVP PILGFYQMKE EEVQLRITIN LKLHESVKNN FEFCEAHIPF361YNRGPITHLE YKTSFGQLEV FREKSLLIWI IGQKFPKSME ISLSGTVTFG AKSHEKQPFD421PICTGETAYL KLHFRILDYT LTGCYADQHS VQVFASGKPK ISAHRKLISS DYYIWNSKAP481APVTYGSLLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
490 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.