Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA5 / Annexin V Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99346-100
Catalog Number: LS-G99346
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin V(ANXA5) (NM_001154), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANX5 | ANXA5 | Anchorin CII | Calphobindin I | CBP-I | ENX2 | PP4 | RPRGL3 | Lipocortin V | Thromboplastin inhibitor | VAC-alpha | Vascular anticoagulant-alpha | PAP-I | Annexin A5 | Annexin V | Annexin-5 | Endonexin II
- NCBI GenBank
- NM_001154
- SwissProt
- P08758
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQVLRGTVT DFPGFDERAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEISAAFKTL61FGRDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTNEKVL TEIIASRTPE121ELRAIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDAGIDEA QVEQDAQALF181QAGELKWGTD EEKFITIFGT RSVSHLRKVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV241VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVMVSRSETD LFNIRKEFRK NFATSLYSMI301KGDTSGDYKK ALLLLCGEDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
320 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.