Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA4 / Annexin IV Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118751-100
Catalog Number: LS-G118751
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin IV(ANXA4) (NM_001153), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 35-beta calcimedin | ANX4 | ANXA4 | Annexin A4 | Annexin IV | Annexin-4 | Chromobindin-4 | Endonexin I | Lipocortin IV | p32.5 | PIG28 | Proliferation-inducing gene 28 | Protein II | ZAP36 | p33/41 | PAP-II | PP4-X
- Origin Species
- Human
- NCBI GenBank
- NM_001153
- SwissProt
- P09525
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMATKGGTV KAASGFNAME DAQTLRKAMK GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS61TIGRDLIDDL KSELSGNFEQ VIVGMMTPTV LYDVQELRRA MKGAGTDEGC LIEILASRTP121EEIRRISQTY QQQYGRSLED DIRSDTSFMF QRVLVSLSAG GRDEGNYLDD ALVRQDAQDL181YEAGEKKWGT DEVKFLTVLC SRNRNHLLHV FDEYKRISQK DIEQSIKSET SGSFEDALLA241IVKCMRNKSA YFAEKLYKSM KGLGTDDNTL IRVMVSRAEI DMLDIRAHFK RLYGKSLYSF301IKGDTSGDYR KVLLVLCGGD DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
321 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.