Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA2 / Annexin A2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99254-100
Catalog Number: LS-G99254
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin A2(ANXA2) (NM_001002857), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Annexin A2 | ANXA2 | Annexin-2 | ANX2 | Calpactin I heavy chain | Chromobindin-8 | CAL1H | Calpactin I heavy polypeptide | Chromobindin 8 | LPC2 | LIP2 | Lipocortin II | p36 | LPC2D | PAP-IV | Protein I | Annexin II | ANX2L4 | Calpactin-1 heavy chain
- NCBI GenBank
- NM_001002858
- SwissProt
- P07355
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL61TNRSNAQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG121LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGDFRKL MVALAKGRRA181EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVPHLQKVFD RYKSYSPYDM241LESIRKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMASRSEVDM301LKIRSEFKRK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
339 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.