Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA10 / Annexin A10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100987-100
Catalog Number: LS-G100987
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin A10(ANXA10) (NM_007193), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Annexin 14 | Annexin-14 | Annexin A10 | Annexin-10 | ANX14 | ANXA10
- Origin Species
- Human
- NCBI GenBank
- NM_007193
- SwissProt
- Q9UJ72
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFCGDYVQGT IFPAPNFNPI MDAQMLGGAL QGFDCDKDML INILTQRCNA QRMMIAEAYQ61SMYGRDLIGD MREQLSDHFK DVMAGLMYPP PLYDAHELWH AMKGVGTDEN CLIEILASRT121NGEIFQMREA YCLQYSNNLQ EDIYSETSGH FRDTLMNLVQ GTREEGYTDP AMAAQDAMVL181WEACQQKTGE HKTMLQMILC NKSYQQLRLV FQEFQNISGQ DMVDAINECY DGYFQELLVA241IVLCVRDKPA YFAYRLYSAI HDFGFHNKTV IRILIARSEI DLLTIRKRYK ERYGKSLFHD301IRNFASGHYK KALLAICAGD AEDYTRTRPL EQKLISEEDL AANDILDYKD DDDKV
355 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.