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ANKS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107497-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKS3 (NM_133450), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKS3 | KIAA1977
Origin Species
Human
NCBI GenBank
NM_133450
SwissProt
Q6ZW76

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSELSDEASE PELLNRSLSM WHGLGTQVSG EELDVPLDLH TAASIGQYEV VKECVQRREL
61DLNKKNGGGW TPLMYASYIG HDTIVHLLLE AGVSVNVPTP EGQTPLMLAS SCGNESIAYF
121LLQQGAELEM KDIQGWTALF HCTSAGHQHM VRFLLDSGAN ANVREPICGF TPLMEAAAAG
181HEIIVQYFLN HGVKVDARDH SGATARMLAK QYGHMKIVAL MDTYSPSLPK SLYRSPEKYE
241DLSSSDESCP APQRQRPCRK KGVSIHEGPR ALARITGIGL GGRAPRPRYE QAPPRGYVTF
301NSSGENPLEE EGLCCRDVTS PINERDVESS SSSSSREEHA FCANLGPVQS SSSSEGLARA
361QGLSSEASVE SNEDSDHACK SSARKQAKSY MKTKNPDSQW PPRAATDREG FLAESSPQTQ
421RAPYSGPQDL AALLEQIGCL KYLQVFEEQD VDLRIFLTLT ESDLKEIGIT LFGPKRKMTS
481AIARWHSSAR PPGDALELAY ADRLEAEMQE LAIQLHKRCE EVEATRGQVC QEQELRAVVE
541SCLLEQDRAR EDLQARLRET WALARDAALV LDQLRACQAE LSSRVRQDQP PGAATLGLAV
601PPADSKGWQA SLQAMSLPEL SGALEDRVRE MGQALCLVTQ SLEKLQVLNG KKWRET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

656 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.