Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANKS1A / ODIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113381-100
Catalog Number: LS-G113381
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKS1A (NM_015245), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKS1 | ANKS1A | KIAA0229 | Odin
- Origin Species
- Human
- NCBI GenBank
- D86982
- SwissProt
- Q92625
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 122.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKEQELLEA ARTGHLPAVE KLLSGKRLSS GFGGGGGGGS GGGGGGSGGG GGGLGSSSHP61LSSLLSMWRG PNVNCVDSTG YTPLHHAALN GHKDVVEVLL RNDALTNVAD SKGCYPLHLA121AWKGDAQIVR LLIHQGPSHT RVNEQNNDNE TALHCAAQYG HTEVVKVLLE ELTDPTMRNN181KFETPLDLAA LYGRLEVVKM LLNAHPNLLS CNTKKHTPLH LAARNGHKAV VQVLLDAGMD241SNYQTEMGSA LHEAALFGKT DVVQILLAAG TDVNIKDNHG LTALDTVREL PSQKSQQIAA301LIEDHMTGKR STKEVDKTPP PQPPLISSMD SISQKSQGDV EKAVTELIID FDANAEEEGP361YEALYNAISC HSLDSMASGR SSDQDSTNKE AEAAGVKPAG VRPRERPPPP AKPPPDEEEE421DHIDKKYFPL TASEVLSMRP RIHGSAAREE DEHPYELLLT AETKKVVLVD GKTKDHRRSS481SSRSQDSAEG QDGQVPEQFS GLLHGSSPVC EVGQDPFQLL CTAGQSHPDG SPQQGACHKA541SMQLEETGVH APGASQPSAL DQSKRVGYLT GLPTTNSRSH PETLTHTASP HPGGAEEGDR601SGARSRAPPT SKPKAELKLS RSLSKSDSDL LTCSPTEDAT MGSRSESLSN CSIGKKRLEK661SPSFASEWDE IEKIMSSIGE GIDFSQERQK ISGSRTLEQS VGEWLESIGL QQYESKLLLN721GFDDVHFLGS NVMEEQDLRD IGISDPQHRR KLLQAARSLP KVKALGYDGN SPPSVPSWLD781SLGLQDYVHS FLSSGYSSID TVKNLWELEL VNVLKVQLLG HRKRIIASLA DRPYEEPPQK841PPRFSQLRCQ DLLSQTSSPL SQNDSCTGRS ADLLLPPGDT GRRRHDSLHD PAAPSRAERF901RIQEEHREAK LTLRPPSLAA PYAPVQSWQH QPEKLIFESC GYEANYLGSM LIKDLRGTES961TQDACAKMRK STEHMKKIPT IILSITYKGV KFIDASNKNV IAEHEIRNIS CAAQDPEDLC1021TFAYITKDLQ TSHHYCHVFS TVDVNLTYEI ILTLGQAFEV AYQLALQAQK SRATGASAAE1081MIETKSSKPV PKPRVGVRKS ALEPPDMDQD AQSHASVSWV VDPKPDSKRS LSTNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1134 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.