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ANKRD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD7 antibodies →
$504.00each
Catalog Number: LS-G111391-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD7 (NM_019644), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD7 | Ankyrin repeat domain 7 | TSA806 | Testis-specific protein TSA806
Origin Species
Human
NCBI GenBank
NM_019644
SwissProt
Q92527

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
28.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKLFSFWKR KNETRSQGYN LREKDLKKLH RAASVGDLKK LKEYLQIKKY DVNMQDKKYR
61TPLHLACANG HTDVVLFLIE QQCKINVRDS ENKSPLIKAV QCQNEDCATI LLNFGADPDL
121RDIRYNTVLH YAVCGQSLSL VEKLLEYEAD LEAKNKDGYT PLLVAVINNN PKMVKFLLEK
181GADVNASDNY QRTALILAVS GEPPCLVKLL LQQGVELCYE GIVDSQLRNM FISMVLLHRY
241PQFTASHGKK KHAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

254 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.