Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANKRD6 / Diversin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113576-100
Catalog Number: LS-G113576
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD6 (NM_014942), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKRD6 | Diversin | Ankyrin repeat domain 6 | KIAA0957
- Origin Species
- Human
- NCBI GenBank
- AB023174
- SwissProt
- Q9Y2G4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQQDAVAAL SERLLVAAYK GQTENVVQLI NKGARVAVTK HGRTPLHLAA NKGHLPVVQI61LLKAGCDLDV QDDGDQTALH RATVVGNTEI IAALIHEGCA LDRQDKDGNT ALHEASWHGF121SQSAKLLVKA GANVLAKNKA GNTALHLACQ NSHSQSTRVL LLAGSRADLK NNAGDTCLHV181AARYNHLSII RLLLTAFCSV HEKNQAGDTA LHVAAALNHK KVAKILLEAG ADTTIVNNAG241QTPLETARYH NNPEVALLLT KAPQVLRFSR GRSLRKKRER LKEERRAQSV PRDEVAQSKG301SVSAGDTPSS EQAVARKEEA REEFLSASPE PRAKDDRRRK SRPKVSAFSD PTPPADQQPG361HQKNLHAHNH PKKRNRHRCS SPPPPHEFRA YQLYTLYRGK DGKVMQAPIN GCRCEPLINK421LENQLEATVE EIKAELGSVQ DKMNTKLGQM ENKTQHQMRV LDKLMVERLS AERTECLNRL481QQHSDTEKHE GEKRQISLVD ELKTWCMLKI QNLEQKLSGD SRACRAKSTP STCVDQLVVT541AGPAAASDSS PPVVRPKEKA LNSTATQRLQ QELSSSDCTG SRLRNVKVQT ALLPMNEAAR601SDQQAGPCVN RGTQTKKSGK SGPTRHRAQQ PAASSTCGQP PPATGSEQTG PHIRDTSQAL661ELTQYFFEAV STQMEKWYER KIEEARSQAN QKAQQDKATL KEHIKSLEEE LAKLRTRVQK721ENTRTRPLEQKLISEEDLAANDILDYKDDDDKV
722 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.