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ANKRD55 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD55 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD55 antibodies →
$725.00each
Catalog Number: LS-G109875-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD55 (NM_024669), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ankyrin repeat domain 55 | ANKRD55
Origin Species
Human
NCBI GenBank
NM_024669
SwissProt
Q3KP44

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMRQATMDFS TPSVFDQQRG DSSEEVDLTM VYQAASNGDV NALTAVIRED PSILECCDSE
61GCTPLMHAVS GRQADTVKLL LKMGANINMQ DAYGRTSLCL ATYLGWLEGC VSLLRNGAKH
121NIPDKNGRLP LHAATAEPDM RLLTVLLQQS NISEINHQDN EGMTPLHWAA FHNQPQHTQM
181LLKKGADPTL VDKDFKTALH WAVQSGNRIL CSIILSHHQG PSIINYDDES GKTCVHIAAA
241AGFSDIIHEL ARVPECNLQA LDVDDRTPLH WAAAAGKAEC VQSLLELGMD SNLRDINEST
301PLAYALYCGH TACVKLLSQE SRTEPTRPPP SQSSRPQKKE RRFNVLNQIF CKNKKEEQRA
361HQKDPSRDRY REEDTSEVND IITTFDSIVG TNCQEQPGDQ VAMVEFKKKT SDNSKYLLPE
421KKPLARKGLP PIRTQSLPPI TLGNNFLTAS HRATSHAGLS SAPHHMAQRS QKSRSEQDLL
481NNRTGCQMLL DNPWKSDSNQ VFSYKVWTVS SSDKLLDRLL SVRPGHQEVS VPPHLRHLHN
541PSSGQNFQHL SPNRHKIRDL PFTRNNLAPL PDQKFLSGEP LRTNRVLPAI PSQRRHSTAA
601EESEHSANPT SDEN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

614 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.