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ANKRD53 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD53 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD53 antibodies →
$504.00each
Catalog Number: LS-G124390-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD53 (NM_001115116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD53 | Ankyrin repeat domain 53
Origin Species
Human
NCBI GenBank
NM_001115116
SwissProt
Q8N9V6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MASAGSTARR AGSGSWHSER GEGRGARPQP TPSGSMQQAN KVSLKATWTD AESKQPSQPL
61PDLADHLSAQ ATALARPRRP ASLTPPRADP SPSKESDQTA IDQTAIGSYY QLFAAAVGNV
121EWLRFCLNQS LREIPTDDKG FTAIHFAAQW GKLACLQVLV EEYKFPVDLL TNNSQTPLHL
181VIHRDNTTVA LPCIYYLLEK GADLNAQTCN GSTPLHLAAR DGLLDCVKVL VQSGANVHAQ
241DAMGYKPIDF CKIWNHRACA RFLKDAMWKK DKKDFAREMT KMKMFKSQLT LMEHNYLIEY
301QKEHKILREA AIRKWLHGKL HPGHSLVSNT KQARATALSK TPEQRESQRS RSFHPSVDAR
361LQCIPQPTEM PKPIYRKPTV KRPTMWNVSN NPARPPTTQI SHSQGIRLGV HPDPTPEHDF
421SSFLEVRPDG HGGARLHTVD GHWVAPVPRL PFEVLLRMLY PRVWPYRMKV PQGFYPISMR
481EVPRKRHLGD NTFWTDTLAM NLRDTFDEAF LAAVRSHQGL PTLPSPQTNP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.