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ANKRD52 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD52 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD52 antibodies →
$725.00each
Catalog Number: LS-G127499-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANR52(ANKRD52) (NM_173595), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD52 | Ankyrin repeat domain 52 | CVWG5837 | PP6-ARS-C | Ankyrin repeat domain 33
Origin Species
Human
NCBI GenBank
NM_173595
SwissProt
Q8NB46

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGILSITDQP PLVQAIFSRD VEEVRSLLSQ KENINVLDQE RRTPLHAAAY VGDVPILQLL
61LMSGANVNAK DTLWLTPLHR AAASRNEKVL GLLLAHSADV NARDKLWQTP LHVAAANRAT
121KCAEALAPLL SSLNVADRSG RSALHHAVHS GHLETVNLLL NKGASLNVCD KKERQPLHWA
181AFLGHLEVLK LLVARGADLG CKDRKGYGLL HTAAASGQIE VVKYLLRMGA EIDEPNAFGN
241TALHIACYLG QDAVAIELVN AGANVNQPND KGFTPLHVAA VSTNGALCLE LLVNNGADVN
301YQSKEGKSPL HMAAIHGRFT RSQILIQNGS EIDCADKFGN TPLHVAARYG HELLISTLMT
361NGADTARRGI HDMFPLHLAV LFGFSDCCRK LLSSGQLYSI VSSLSNEHVL SAGFDINTPD
421NLGRTCLHAA ASGGNVECLN LLLSSGADLR RRDKFGRTPL HYAAANGSYQ CAVTLVTAGA
481GVNEADCKGC SPLHYAAASD TYRRAEPHTP SSHDAEEDEP LKESRRKEAF FCLEFLLDNG
541ADPSLRDRQG YTAVHYAAAY GNRQNLELLL EMSFNCLEDV ESTIPVSPLH LAAYNGHCEA
601LKTLAETLVN LDVRDHKGRT ALFLATERGS TECVEVLTAH GASALIKERK RKWTPLHAAA
661ASGHTDSLHL LIDSGERADI TDVMDAYGQT PLMLAIMNGH VDCVHLLLEK GSTADAADLR
721GRTALHRGAV TGCEDCLAAL LDHDAFVLCR DFKGRTPIHL ASACGHTAVL RTLLQAALST
781DPLDAGVDYS GYSPMHWASY TGHEDCLELL LEHSPFSYLE GNPFTPLHCA VINNQDSTTE
841MLLGALGAKI VNSRDAKGRT PLHAAAFADN VSGLRMLLQH QAEVNATDHT GRTALMTAAE
901NGQTAAVEFL LYRGKADLTV LDENKNTALH LACSKGHEKC ALMILAETQD LGLINATNSA
961LQMPLHIAAR NGLASVVQAL LSHGATVLAV DEEGHTPALA CAPNKDVADC LALILSTMKP
1021FPPKDAVSPF SFSLLKNCSI AAAKTVGGCG ALPHGASCPY SQERPGAIGL DGCYSE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1076 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.