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ANKRD50 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD50 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD50 antibodies →
$725.00each
Catalog Number: LS-G111249-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD50 (NM_020337), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ankyrin repeat domain 50 | ANKRD50 | KIAA1223
Origin Species
Human
NCBI GenBank
AB033049
SwissProt
Q9ULJ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
155.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTNPWEEKVC KMAQTSLLQG KQFYCREWVF HKLQHCLQEK SNCCNSAVNA PSLVMNSGNN
61ASGVSGKGAA WGVLLVGGPG SGKTALCTEL LWPSSPASLQ RGLHRQALAF HFCKAQDSDT
121LCVGGFIRGL VAQICRSGLL QGYEDKLRDP AVQSLLQPGE CERNPAEAFK RCVLLPLLGM
181KPPQQSLYLL VDSVDEGCNI TEGEQTSTSL SGTVAALLAG HHEFFPPWLL LLCSARKQSK
241AVTKMFTGFR KISLDDLRKA YIVKDVQQYI LHRLDQEEAL RQHLTKETAE MLNQLHIKSS
301GCFLYLERVL DGVVENFIML REIRDIPGTL NGLYLWLCQR LFVRKQFAKV QPILNVILAA
361CRPLTITELY HAVWTKNMSL TLEDFQRKLD ILSKLLVDGL GNTKILFHYS FAEWLLDVKH
421CTQKYLCNAA EGHRMLAMSY TCQAKNLTPL EAQEFALHLI NSNLQLETAE LALWMIWNGT
481PVRDSLSTLI PKEQEVLQLL VKAGAHVNSE DDRTSCIVRQ ALEREDSIRT LLDNGASVNQ
541CDSNGRTLLA NAAYSGSLDV VNLLVSRGAD LEIEDAHGHT PLTLAARQGH TKVVNCLIGC
601GANINHTDQD GWTALRSAAW GGHTEVVSAL LYAGVKVDCA DADSRTALRA AAWGGHEDIV
661LNLLQHGAEV NKADNEGRTA LIAAAYMGHR EIVEHLLDHG AEVNHEDVDG RTALSVAALC
721VPASKGHASV VSLLIDRGAE VDHCDKDGMT PLLVAAYEGH VDVVDLLLEG GADVDHTDNN
781GRTPLLAAAS MGHASVVNTL LFWGAAVDSI DSEGRTVLSI ASAQGNVEVV RTLLDRGLDE
841NHRDDAGWTP LHMAAFEGHR LICEALIEQG ARTNEIDNDG RIPFILASQE GHYDCVQILL
901ENKSNIDQRG YDGRNALRVA ALEGHRDIVE LLFSHGADVN CKDADGRPTL YILALENQLT
961MAEYFLENGA NVEASDAEGR TALHVSCWQG HMEMVQVLIA YHADVNAADN EKRSALQSAA
1021WQGHVKVVQL LIEHGAVVDH TCNQGATALC IAAQEGHIDV VQVLLEHGAD PNHADQFGRT
1081AMRVAAKNGH SQIIKLLEKY GASSLNGCSP SPVHTMEQKP LQSLSSKVQS LTIKSNSSGS
1141TGGGDMQPSL RGLPNGPTHA FSSPSESPDS TVDRQKSSLS NNSLKSSKNS SLRTTSSTAT
1201AQTVPIDSFH NLSFTEQIQQ HSLPRSRSRQ SIVSPSSTTQ SLGQSHNSPS SEFEWSQVKP
1261SLKSTKASKG GKSENSAKSG SAGKKAKQSN SSQPKVLEYE MTQFDRRGPI AKSGTAAPPK
1321QMPAESQCKI MIPSAQQEIG RSQQQFLIHQ QSGEQKKRNG IMTNPNYHLQ SNQVFLGRVS
1381VPRTMQDRGH QEVLEGYPSS ETELSLKQAL KLQIEGSDPS FNYKKETPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1429 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.