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ANKRD44 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD44 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD44 antibodies →
$725.00each
Catalog Number: LS-G105711-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD44 (NM_153697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD44 | Ankyrin repeat domain 44 | PP6-ARS-B
Origin Species
Human
NCBI GenBank
AA916533

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLIHKTEDVN TLDSEKRTPL HVAAFLGDAE IIELLILSGA RVNAKDNMWL TPLHRAVASR
61SEEAVQVLIK HSADVNARDK NWQTPLHVAA ANKAVKCAEV IIPLLSSVNV SDRGGRTALH
121HAALNGHVEM VNLLLAKGAN INAFDKKDRR ALHWAAYMGH LDVVALLINH GAEVTCKDKK
181GYTPLHAAAS NGQINVVKHL LNLGVEIDEI NVYGNTALHI ACYNGQDAVV NELIDYGANV
241NQPNNNGFTP LHFAAASTHG ALCLELLVNN GADVNIQSKD GKSPLHMTAV HGRFTRSQTL
301IQNGGEIDCV DKDGNTPLHV AARYGHELLI NTLRTSGADT AKCGIHSMFP LHLAALNAHS
361DCCRKLLSSG FEIDTPDKFG RTCLHAAAAG GNVECIKLLQ SSGADFHKKD KCGRTPLHYA
421AANCHFHCIE TLVTTGANVN ETDDWGRTAL HYAAASDMDR NKTILGNAHD NSEELERARE
481LKEKEATLCL EFLLQNDANP SIRDKEGYNS IHYAAAYGHR QCLELLLERT NSGFEESDSG
541ATKSPLHLAA YNGHHQALEV LLQSLVDLDI RDEKGRTALD LAAFKGHTEC VEALINPGAS
601IFVKDNVTKR TPLHASVING HTLCLRLLLE IADNPEAVDV KDAKGQTPLM LAVAYGHIDA
661VSLLLEKEAN VDTVDILGCT ALHRGIMTGH EECVQMLLEQ EVSILCKDSR GRTPLHYAAA
721RGHATWLSEL LQMALSEEDC CFKDNQGYTP LHWACYNGNE NCIEVLLEQK CFRKFIGNPF
781TPLHCAIIND HGNCASLLLG AIDSSIVSCR DDKGRTPLRA AAFADHVECL QLLLRHSAPV
841NAVDNSGKTA LMMAAENGQA GAVDILVNSA QADLTVKDKD LNTPLHLACS KGHEKCALLI
901LDKIQDESLI NEKNNALQT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

919 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.