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ANKRD35 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD35 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD35 antibodies →
$504.00each
Catalog Number: LS-G106893-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD35 (NM_144698), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ankyrin repeat domain 35 | ANKRD35
Origin Species
Human
NCBI GenBank
NM_144698
SwissProt
Q8N283

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKRIFSCSST QVAVERWNRH DQKLLEAVHR GDVGRVAALA SRKSARPTKL DSNGQSPFHL
61AASKGLTECL TILLANGADI NSKNEDGSTA LHLATISCQP QCVKVLLQHG ANEDAVDAEN
121RSPLHWAASS GCASSVLLLC DHEAFLDVLD NDGRTPLMIA SLGGHAAICS QLLQRGARVN
181VTDKNDKSAL ILACEKGSAE VAELLLSHGA DAGAVDSTGH DALHYALHTQ DKALWRHLQQ
241ALSRRRRGGQ RLVQHPDLAS QASPSEPQAG SPPKSSWRAE PEEEQEEKED EDPCSEEWRW
301KYEEERRKVV RLEQELVQKT EECKTQAAAY LDLENQIREQ AQELGVLLSW EPRASGKQGS
361SLRPGGDGME QGCPKDLLAE STQELKKQQQ AAATVNPVLA PKKAEDSAPG KIQYEVHGRS
421QPEEQGPSQS PASETIRKAT GQQLTTNGAQ TFGPDHADQL PAGQKESSQV LGVEPGGTVA
481EPVGPAAMNQ LLLQLREELA AVWREKDAAR GALSRPVMEG ALGTPRAEAA AAAWEKMEAR
541LERVLARLEW AKAGLQVKPE VPSQESREGA LKAAPGSIKQ DEEKEKRVPG ARGEPLGALG
601GEKALGGLAK GQLEKEMSVL RLSNSNLLEE LGELGRERQR LQRELQSLSQ RLQREFVPKP
661EAQVQLQQLR QSVGLLTNEL AMEKEATEKL RKLLASQSSG LRGLWDCLPA DLVGERSAQS
721KAAESLEELR ACISTLVDRH REAQQVLARL QEENQQLRGS LSPCREPGTS LKAPASPQVA
781ALEQDLGKLE EELRAVQATM SGKSQEIGKL KQLLYQATEE VAELRAREAA SLRQHEKTRG
841SLVAQAQAWG QELKALLEKY NTACREVGRL REAVAEERRR SGDLAAQAAE QERQASEMRG
901RSEQFEKTAE LLKEKMEHLI GACRDKEAKI KELLKKLEQL SEEVLAIRGE NARLALQLQD
961SQKNHEEIIS TYRNHLLNAA RGYMEHEVYN ILLQILSMEE E
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1001 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.