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ANKRD20A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD20A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD20A1 antibodies →
$725.00each
Catalog Number: LS-G108960-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD20A1 (NM_032250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD20A1 | Ankyrin repeat domain 20A | ANKRD20A
Origin Species
Human
NCBI GenBank
NM_032250
SwissProt
Q5TYW2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKLFGFGSRR GQTAQGSIDH VYTGSGYRIR DSELQKIHRA AVKGDAAEVE RCLARRSGDL
61DALDKQHRTA LHLACTSGHV QVVTLLVNRK CQIDVCDKEN RTPLIQAVHC QEEACAVILL
121EHGANPNLKD IYGNTALHYA VYSESTSLAE KLLSHGAHIE ALDKDNNTPL LFAIICKKEK
181MVEFLLKKKA SSHAVDRLRR SALMLAVYYD SPGIVNILLK QNIDVFAQDM CGRDAEDYAI
241SHHLTKIQQQ ILEHKKKILK KEKSDVGSSD ESAVSIFHEL RVDSLPASDD KDLNVATKQC
301VPEKVSEPLP GSSHEKGNRI VNGQGEGPPA KHPSLKPSTE VEDPAVKGAV QRKNVQTLRA
361EQALPVASEE EQERHERSEK KQPQVKEGNN TNKSEKIQLS ENICDSTSSA AAGRLTQQRK
421IGKTYPQQFP KKLKEEHDRC TLKQENEEKT NVNMLYKKNR EELERKEKQY KKEVEAKQLE
481PTVQSLEMKS KTARNTPNWD FHNHEEMKGL MDENCILKAD IAILRQEICT MKNDNLEKEN
541KYLKDIKIVK ETNAALEKYI KLNEEMITET AFRYQQELND LKAENTRLNA ELLKEKESKK
601RLEADIESYQ SRLAAAISKH SESVKTERNL KLALERTRDV SVQVEMSSAI SKVKAENEFL
661TEQLSETQIK FNALKDKFRK TRDSLRKKSL ALETVQNDLS QTQQQTQEMK EMYQNAEAKV
721NNSTGKWNCV EERICHLQRE NAWLVQQLDD VHQKEDHKEI VTNIQRGFIE SGKKDLVLEE
781KSKKLMNECD HLKESLFQYE REKTEGVVSI KEDKYFQTSR KTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

823 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.