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ANKRD13D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD13D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD13D antibodies →
$725.00each
Catalog Number: LS-G102768-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD13D (NM_207354), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD13D
Origin Species
Human
SwissProt
Q6ZTN6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MVQLVLQYRD YQRATQRLAG IPELLNKLRQ APDFYVEMKW EFTSWVPLVS KMCPSDVYRV
61WKRGESLRVD TSLLGFEHMT WQRGRRSFIF KGQEAGALVM EVDHDRQVVH VETLGLTLQE
121PETLLAAMRP SEEHVASRLT SPIVSTHLDT RNVAFERNKC GIWGWRSEKM ETVSGYEAKV
181YSATNVELVT RTRTEHLSDQ DKSRSKAGKT PFQSFLGMAQ QHSSHTGAPV QQAASPTNPT
241AISPEEYFDP NFSLESRNIG RPIEMSSKVQ RFKATLWLSE EHPLSLGDQV TPIIDLMAIS
301NAHFAKLRDF ITLRLPPGFP VKIEIPLFHV LNARITFSNL CGCDEPLSSV WVPAPSSAVA
361ASGNPFPCEV DPTVFEVPNG YSVLGMERNE PLRDEDDDLL QFAIQQSLLE AGTEAEQVTV
421WEALTNTRPG ARPPPQATVY EEQLQLERAL QESLQLSTEP RGPGSPPRTP PAPGPPSFEE
481QLRLALELSS REQEERERRG QQEEEDLQRI LQLSLTEH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.