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ANKRD13C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD13C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD13C antibodies →
$504.00each
Catalog Number: LS-G109379-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD13C (NM_030816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD13C | Ankyrin repeat domain 13C | DJ677H15.3 | RP4-677H15.5 | DKFZP566D1346
Origin Species
Human
NCBI GenBank
NM_030816
SwissProt
Q8N6S4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTGEKIRSLR RDHKPSKEEG DLLEPGDEEA AAALGGTFTR SRIGKGGKAC HKIFSNHHHR
61LQLKAAPASS NPPGAPALPL HNSSVTANSQ SPALLAGTNP VAVVADGGSC PAHYPVHECV
121FKGDVRRLSS LIRTHNIGQK DNHGNTPLHL AVMLGNKECA HLLLAHNAPV KVKNAQGWSP
181LAEAISYGDR QMITALLRKL KQQSRESVEE KRPRLLKALK ELGDFYLELH WDFQSWVPLL
241SRILPSDACK IYKQGINIRL DTTLIDFTDM KCQRGDLSFI FNGDAAPSES FVVLDNEQKV
301YQRIHHEESE METEEEVDIL MSSDIYSATL STKSISFTRA QTGWLFREDK TERVGNFLAD
361FYLVNGLVLE SRKRREHLSE EDILRNKAIM ESLSKGGNIM EQNFEPIRRQ SLSPPPQNTI
421TWEEYISAEN GKAPHLGREL VCKESKKTFK ATIAMSQEFP LGIELLLNVL EVVAPFKHFN
481KLREFVQMKL PPGFPVKLDI PVFPTITATV TFQEFRYDEF DGSIFTIPDD YKEDPSRFPD
541L
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

541 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.