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ANKRD13B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKRD13B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKRD13B antibodies →
$504.00each
Catalog Number: LS-G106318-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD13B (NM_152345), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD13B | Ankyrin repeat domain 13B
Origin Species
Human
SwissProt
Q86YJ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70 kda
Protein Type
Lysate
Amino Acid Sequence
1MIPANASARK GPEGKYPLHY LVWHNRHREL EKEVRAGQVD IEQLDPRGRT PLHLATTLGH
61LECARVLLAH GADVGRENRS GWTVLQEAVS TRDLELVQLV LRYRDYQRVV KRLAGIPVLL
121EKLRKPQDFY VEMKWEFTSW VPLVSKICPS DTYKVWKSGQ NLRVDTTLLG FDHMTWQRGN
181RSFVFRGQDT SAVVMEIDHD RRVVYTETLA LAGQDRELLL AAAQPTEEQV LSRLTAPVVT
241TQLDTKNISF ERNKTGILGW RSEKTEMVNG YEAKVYGASN VELITRTRTE HLSEHHKGKV
301KGCKTPLQSF LGIAEQHGGP QNGTLITQTL SQANPTAITA EEYFNPNFEL GNRDMGRPME
361LTTKTQKFKA KLWLCEEHPL SLCEQVAPII DLMAVSNALF AKLRDFITLR LPPGFPVKIE
421IPIFHILNAR ITFGNLNGCD EPVPSVRGSP SSETPSPGSD SSSVSSSSST TSCRGCEISP
481ALFEAPRGYS MMGGQREAAT RDDDDDLLQF AIQQSLLEAG SEYDQVTIWE ALTNSKPGTH
541PMSYEGRRQD RSAPPTPQRQ PAPPASVPSP RPSSGPGSGG HVFRSYDEQL RLAMELSAQE
601QEERRRRARQ EEEELERILR LSLTEQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.